Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide
نویسندگان
چکیده
A simple and economical minibioreactor-gas collector system for determination of hydrogen sulfide produced by a microorganism was designed. The detection of hydrogen sulfide was based on the reaction between the hydrogen sulfide in the gas stream from the culture, and a lead acetate solution 0.090 mol/L, contained in a tube gas collector; the conductimetric back titration of lead excess was made, and hydrogen sulfide was quantified indirectly, being the detection limit 0.5 μmol. The developed system was applied using Tsukamurella paurometabola DSM 20162 as a model, being the amount of hydrogen sulfide produced, 1.2 μmol in 24 hrs.
منابع مشابه
Modeling and Removal of Hydrogen Sulfide from Biogas Produced by Anaerobic Digestion
Anaerobic digestion can be used to convert organic waste into energy not only to provide renewable energy, but also reduce greenhouse gases. During the anaerobic digestion process, biogas is produced, which can be used for heating and electricity generation. The produced biogas contains methane and some other gases, the most destructive of which is hydrogen sulfide gas. If hydrogen sulfide gas ...
متن کاملHydrogen Sulfide and Sulfuric Acid Estimation Techniques SULFIDE GENERATION IN SEWERS
The root cause of odor and corrosion in collection systems is sulfide, which is produced from sulfate by bacteria residing in a slime layer on the submerged portion of sewer pipes and structures. Once released from the wastewater as hydrogen sulfide gas, odor and corrosion problems begin. Another type of bacteria utilizes hydrogen sulfide gas to produce sulfuric acid that causes the destruction...
متن کاملHydrogen sulfide induces direct radical-associated DNA damage.
Hydrogen sulfide (H(2)S) is produced by indigenous sulfate-reducing bacteria in the large intestine and represents an environmental insult to the colonic epithelium. Clinical studies have linked the presence of either sulfate-reducing bacteria or H(2)S in the colon with chronic disorders such as ulcerative colitis and colorectal cancer, although at this point, the evidence is circumstantial and...
متن کاملHigh rate electrochemical sulfide removal from synthetic feed and real domestic wastewater
The microbial reduction of sulfate to hydrogen sulfide causes sewer pipe corrosion, one of the major issues in the water infrastructure [1]. Oxygen injection is presently considered as an attractive option for sulfide abatement in sewer systems. Oxygen can both inhibit the activity of sulfate reducing bacteria (SRB) and oxidize the sulfide that has been produced [2]. It is less expensive than m...
متن کاملHydrogen sulfide: a toxic gas produced by dissimilatory sulfate and sulfur reduction and consumed by microbial oxidation.
Sulfur is an essential element for the synthesis of cysteine, methionine, and other organo-sulfur compounds needed by living organisms. Additionally, some prokaryotes are capable of exploiting oxidation or reduction of inorganic sulfur compounds to energize cellular growth. Several anaerobic genera of Bacteria and Archaea produce hydrogen sulfide (H2S), as a result of using sulfate (SO(4)(2 -) ...
متن کامل